Feed micronutrients are essential nutrients that play an important role in providing vitamins, minerals, and other micronutrients to enhance the overall animal growth and performance of livestock and poultry. Moreover, micronutrients are vital for metabolic processes, immunity, and reproductive functions in animals. As per FACTMR, the global animal feed micronutrient market is expected to reach a valuation of over US$ 2.5 Bn by 2027.

Author:  S. N. Jha
AVP and Principal Consultant
Fact.MR

Recent key trends in the feed micronutrients market:

  • Focus on sustainable and organic solutions: Consumers are increasingly aware of the environmental and ethical implications of the products they use. Major players in the market are focusing on sustainable and organic products due to growing consumer awareness. In addition to this, key companies also exploring the options of environmentally friendly and ethically sourced alternatives. This ultimately minimizes the ecological footprint of material extraction and processing. These initiatives also help companies improve their public image and brand image. Consumers also prefer brands that align with their values, and sustainability can foster long-term brand loyalty.
  • Inclination towards personalized nutrition solutions: Another key trend in the market is to offer personalized nutrition solutions. Major players in the market adapt their offerings to the specific nutritional needs of animal species. Different animal species may have different nutritional requirements based on various factors such as age, physiological conditions, weight, etc. Market players have been increasingly offering specific nutritional profiles that optimize the health, growth, and production of each species.
  • Adoption of digital technologies: Key players in the micronutrient market have been widely adopting digital technologies. Key companies have adopted advanced analytics and digital platforms such as precision agriculture and data analytics to optimize feed formulas and improve animal nutrition efficiency. Moreover, farmers typically use data-driven insights to optimize livestock production. Moreover, based on inputs from real-time data, satellite images, and sensor data, farmers can use precision farming practices that involve accurate usage of water, pesticides, etc.
    • For example, Alltech is a global company headquartered in Kentucky, US with operations in animal feed, brewing, and distilling. Using data analytics for precision nutrition, the company has been focusing on technologies to improve feed efficiency and animal health.
    • Devenish Nutrition is an agri-technology company based in Belfast and is known for its expertise in animal nutrition. The company has adopted innovations in precision nutrition, including the use of data analytics and technology to optimize feed formulations and improve animal health.
  • Trace Mineral Optimization: Trace Minerals are the core part of animal diet to optimize well-being, health, and performance. Trace mineral optimization is a key trend due to increasing awareness among consumers about animal health & nutrition. Optimizing nutritional micronutrients requires careful management of the concentrations and forms of micronutrients needed to improve overall animal health, performance, and reproduction. Trace elements, including zinc, copper, selenium, and manganese, play a crucial role in various metabolic processes in animals and the body.

Market Challenges:

  • Key companies in the market are facing regulatory constraints due to stringent regulations and approval processes for feed additives including micronutrients. Moreover, meeting regulatory standards in different regions and complying with evolving regulations can be time-consuming and costly.
  • Besides this, the cost of producing and incorporating high-quality micronutrients into animal feed formulations can be a limiting factor, especially for smaller and medium-sized farms. Cost considerations may impact the adoption of advanced micronutrient-enriched feeds.
  • Apart from this, fluctuations in the prices of raw materials used in the production of micronutrients can affect the overall cost of manufacturing. Unpredictable price variations may impact profit margins for manufacturers and, consequently, the pricing of micronutrient-enriched feeds.

Key strategies:

The market is moderately consolidated with key players such as Tanke Biosciences, Vamso Biotec Pvt. Ltd, Archer Daniels Midland, and Pancosma SA occupy the majority of the shares. To establish a strong market share, key companies in the market are focusing on the following key strategies:

  • To expand their market share, key players have been entering new geographical regions. Companies establish partnerships, and collaborations, setting up distribution networks in emerging markets.
    • Nutreco, a global animal nutrition and fish feed company, implemented a strategy to expand its market presence in Latin America, a region with a growing demand for animal protein and a burgeoning livestock industry. Nutreco has acquired local companies and the key is to expand and strengthen its animal nutrition business in Latin America.
  • Moreover, key companies are also offering customized feed solutions tailored to the nutritional needs of different animal species. This strategy involves understanding and addressing the unique requirements of various livestock and poultry.
    • Cargill, a global agribusiness and food company, operates a division called Provimi, which focuses on animal nutrition. Provimi offers a range of customized feed solutions designed to meet the specific nutritional requirements of various animal species, including livestock and poultry.

Mr. S.N. Jha is the principal consultant at Fact.MR, a leading provider of market research, consulting, and data services. With over a decade of experience at Fact.MR, Mr. Jha has provided invaluable insights and strategic direction to more than 1500 clients. His analytical mind and eye for detail have established him as an expert in gleaning actionable intelligence from complex data sets. Clients value his ability to translate market trends into cogent recommendations that drive growth.

Author Contact Details:
S. N. Jha | AVP and Principal Consultant
D: +353-1-4434-232 Tel: +91 783 8476 404
E-mail: shambhu@factmr.com | Web: www.factmr.com

NUQO is pleased to announce the opening of its new affiliate in India, marking a significant milestone for the company. In the last few years, NUQO’s technologies have experienced rapid expansion globally and gained swift recognition in the market in recent years.

Headquartered in Europe, NUQO is a feed additives company and a pioneer in developing technologies based on the micro-encapsulation of metabolites from plants and seaweed (phytogenics and phycogenics). The company has established new standards to produce feed additives for animal nutrition, leveraging unique expertise in manufacturing technologies to produce more active, stable, and effective products, along with a broad knowledge of natural extracts & innovative molecules. Its groundbreaking work has been acknowledged over the last few years through participation in various well-known scientific congresses and publications.

Mr. Ewenn Helary, CEO , NUQO
Mr. Ewenn Helary,
CEO , NUQO

In recent years, NUQO has expanded its distribution network globally and established regional hubs, including affiliates in Thailand and, more recently, in Mexico. Today, NUQO is proud to announce the opening of NUQO Animal Nutrition India Pvt Ltd. Mr. Ewenn Helary, CEO of NUQO, views this development as a remarkable achievement: “We are thrilled to launch Nuqo in India, a large yet demanding market. Two key factors enabled this launch: our offering of technologies with exclusive features that create genuine value for customers, and the opportunity to onboard exceptional colleagues. This milestone underscores the merit of our strategic approach and our ability to execute it successfully as a team.”

With the launch of Nuqo India, NUQO’s dedicated team in India, led by Mr. Neeraj Kumar Srivastava, brings extensive industry experience and local market insights to drive the company’s growth and meet the evolving needs of Indian customers.

Neeraj Kumar Srivastava
Managing Director
NUQO Animal Nutrition India Pvt. Ltd.

With a robust background in the Animal Health and Nutrition industry, Mr. Srivastava has served as Managing Director for major industry players in South & Southeast Asia. He also held the prestigious position of Chairman of CLFMA, a prominent livestock association in India, from 2020 to 2022. In addition, he holds board positions in esteemed industry associations, demonstrating his dedication to industry growth and development. Mr. Srivastava remarked, “Throughout my career, I have cultivated an extensive network and deep market knowledge in India and neighboring countries. I have played pivotal roles in the development of major businesses in animal nutrition within Indian Subcontinent. I strongly believe in the value of Nuqo’s technologies and our team’s capability to deliver these solutions sustainably and effectively to Indian market. Our objective is to address the key needs of the market & our customers, and we believe that NUQO’s science & research based technologies are ideally suited for this purpose.”

Mr. Guillaume Etave, Global Sales Director, Nuqo
Mr. Guillaume Etave,
Global Sales Director, Nuqo

In summary, the launch of Nuqo India represents a significant step forward in NUQO’s global expansion efforts, following years of impressive growth. Mr. Guillaume Etave, Global Sales Director of Nuqo, emphasized the importance of this development: “The establishment of this subsidiary enhances our local presence in India, allowing us to better serve our customers in this rapidly growing market and address their specific needs. It also strengthens our capability to form strong strategic partnerships and fully leverage the unique business opportunities presented by this dynamic market. We are delighted to welcome Mr. Srivastava who joins our team to lead this new initiative” Mr. Etave concluded: “This strategic expansion underscores the relevance of Nuqo’s approach, demonstrating NUQO’s ongoing dedication to meeting customer demands worldwide and seizing opportunities in key emerging markets”.

For more information: neeraj.srivastava@nuqo.in or info@nuqo.eu

Synopsis: Heart attacks are the results of failure of the heart muscles’ function due to poor blood circulation to the heart muscles. This happens due to the one or more blocks in the coronary artery function which supplies blood to the heart muscles to function. These heart muscles are the involuntary muscles performing’ a vital function to help the heart pumping blood to the whole body on non-stop performance of 24×7.

Dr. Ram Moorthy D, , Author Pic_EB
Dr. Ram Moorthy D
CEO, Geenat
E: rammoorthyd@gmail.com
W: www.geenat.co.in
Phone: +91 93446 42382

CARDIAC ARREST in Chicken (Sudden Death Syndrome – SDS)

When two or more heart attacks occur within a short duration it becomes a ‘Cardiac Arrest’ an acute syndrome in which the heart suddenly stops functioning and the bird collapses immediately. In a nutshell heart attack gives second chance for survival; however cardiac arrest leaves no chance. Cardiac arrest (Acute Syndrome) can happen in a healthy bird without prior attacks too.

Such acute incidents (Cardiac Arrest) in Poultry are called ‘Sudden Death Syndrome’ in which a healthy bird dies without any clinical signs or symptoms and/or without any infection.

Postmortem examination of the major organs and the body parts of the chicken will be quite normal. Cardiac arrest or Sudden Death Syndromes are common in broiler birds in comparison to commercial layer birds due to high fat% in their feed.

Chicken Heart

The chicken heart has quite similar anatomy and function as that of mammals including humans with two atria and two ventricles (four chambers) and pumping the oxygenated blood to the whole body and involving in the gaseous exchange.

Factors ‘involved in the Heart Attack and Cardiac Arrest in Chicken

  • Biological stress – Today’s chicken has the evolution of genetic goals
  • Environmental Temperature – Peak weather conditions especially summer.

Yo-Yo Chicken of 2024. Evolution of Genetics with Production goals

Today’s Yo-Yo chickens are genetically evolved to have less feed and deliver more commercial results than yesteryears relaxed birds.

The current days genetically evolved chickens with high production goals in order to deliver the quick results, have ‘inbuilt stress’ within the birds which is inevitable but to sustain.

Biological Stress

The Biological Stress secrets cortisol the stress hormone which leads to many complications in the birds including triggered adrenaline gland leads to heart attack and/or cardiac arrest, sudden death.

Biological stress leads to produce the stress hormone – CORTISOL

Cortisol the stress hormone lowers the body function including low feed intake.

Cortisol Triggers Adrenaline Gland in chicken

Cortisol the stress Hormone triggers Adrenaline Gland to secrete Adrenaline. Adrenaline lowers many body functions, importantly lowers metabolism & increases the body heat, thus increases the heart beats which results in heart attacks and or CARDIAC ARREST (SDS).

Production Goals Produces Bio-Stress & Cortisol Triggers Hypothalamus

Cortisol – The stress hormone, triggers hypothalamus which regulates the birds’ body temperature. Triggered hypothalamus imbalances Chicken’s body temperature and body temperature shoots up resulting in panting and heart attack and/or cardiac arrest – Sudden death syndrome.

During extreme weather conditions, chicken’s blood pH becomes acidic as chicken lacks sweat glands like mammals to reduce the body temperature. It means the blood level Co2 will be higher than Oxygen. Thus, Acidic blood increases the adrenaline level leads to increased heart beats – heart attack & cardiac arrest.

How to control/minimise the Stress and Heart Attacks

It’s quite challenging to minimise the stress levels in today’s poultry however it’s very much possible.

The stress levels in chicken will be high during summer and winter season as the most of the poultry farms are in open environmental system exposed to extreme heat/cold.

EC shed poultry farms are very rare in Asia and Middle East region as the not announced or unplanned ‘power cuts’ is very common and maintaining the farm’s electricity with diesel generator will be additional cost.

Solar Panel based poultry farms will be futuristic with today’s aggressive solar energy promotion.

Poultry farmers’ can continue their regular practices to reduce or increase the farm temperature externally as per their regular consultants’ advice.

Poultry Feed Formulation

A bit Less Crude Protein levels balanced with Amino Acids is the key factor.

Potassium & Crude Fat% – The important factors to avoid heart attacks or SDH in chicken.

Potassium levels are important for bird’s healthy heart. The given statistics can give the handful information for the potassium level and Fat% to be maintained in the Poultry feed formulation to prevent SDS especially in Broiler Chicken.

Egg production parameters

Though fat% is not an issue in commercial layers, it’s wise to include Choline Chloride in the old flocks that are above 50 weeks. Early morning feeding is important to maintain the birds’ energy level for the rest of the day.

Vitamin E, Biotin and B3 Niacin can be top dressed to reduce the Heart Attacks in Chicken

Table: Vitamin E, Biotin and B3 Niacin

Inclusion of Antioxidants

Synthetic antioxidants can help to prevent heart attacks or SDS in Chicken.

Like BHA – Butylated hydroxy anisole, BHT – Butylated hydroxytoluene, Propyl gallate (PG) and TBHQ – Tert-butyl hydroquinone etc. Though the synthetic antioxidants effect is fast and accurate we need to limit their usage due to certain side effects arise due to their long-term usage.

Plant based Antioxidants – Dry Amla extract can be used @ 500 g/MT or as per the consultants’ advice.

Antioxidants can be used as per suppliers’ dosage claim.

Oral Supplements (Electrolytes) through drinking water

We need to maintain the Electrolytes Balance either through drinking water or through feed.

Supplementing electrolytes is essential during Summer & Winter.

Vitamins & Trace Mineral Premixes through feed can be increased by 20 to 30% to the regular dosage

Diluted Butter Milk can be given through drinking water during Summer

Clean & diluted ‘butter milk’ can be given through drinking water @ 20 ml/100 birds

The diluted butter milk should be clean and fresh, without fat molecules. Please note that the diluted butter milk is easy to execute at deep litter farms; to execute in Pipe line system, it should be filtered thoroughly to avoid ‘nipple chocking’ or else can choose the electrolytes with B complex through feed.

Summary:

To the increasing human population and updated life style everyone needs healthy food. Poultry industry is one of the promising industries to feed food challenges that humans are exposed today.

As the demand multi-folds, biotech solutions and evolving genetical goals of the chicken to produce more in less space is must and urgent.

Poultry Industry can assure a healthy food option through chicken meat and eggs with the consistency in taste and top- quality hygiene at 24 x 7.

Hence, every bird saved is every bird produced.


Previous Article by author: Prevention Of CRD & C-CRD – Essential In Poultry

Common Egg Shell Deformities, Discolouration Issues and Probable Reasons

Dr. B.C Dutta
Dr B C Dutta (Author),
Poultry Consultant
Member – Poultry TRENDS Editorial Board
www.drbcdutta.com

Egg production parameters

15 common Egg Shell deformities in respect of structure and colour as under:

Pale eggs1. Pale Shelled Eggs
Reasons:
a) Infections like Infectious Bronchitis & EDS
b) Old Age Hen
c) High Stress in flock
d) Toxic Effect of Drug like Sulphonamides & Nicarbazine

 

 

Pink or Liliac Eggs2. Pink or Liliac Eggs
Reasons:
a) High Stress in Flock
b) Excess Dietary Calcium

 

 

 

Dirty Eggs3. Dirty Eggs
Reasons:
a) Poor Gut Health
b) High volume of indigestible ingredients in Feed
c) Enteritis
d) Electrolyte Imbalance

 

 

Shell less egg4. Shell-less Egg
Reasons:
a) Disease like Avian Influenza, ND, IB & EDS
b) Immature Shell Gland
c) Nutrients like Ca, P, Mn or Vitamin D deficiency

 

 

 

5. Bloody Eggs
Reasons:
a) Rupture of blood vessels inside Fallopian tube wall
b) Rupture of blood vessels on Yolk surface
c) Deficiency of Vit A or Vit K
d) Large size in Egg in Old hen causing stretching and pressure on the lower area, may lead to bleeding
e) Egg may broke inside hen’s body & cause bleeding

 

Soft Shell Eggs6. Soft Shell Eggs
Reasons:
a) Excess Phosphorus in Feed
b) Heat Stress
c) Old Age of Hen
d) Electrolyte Imbalance

 

Cracked Egg7. Cracked Egg
Reasons:
a) Calcium & Vit D deficiency
b) Heat Stress
c) Old Age of hens
d) Electrolyte Imbalance
e) Mycotoxins

 

Corrugated Eggs8. Corrugated Eggs
Reasons:
a) Calcium, Copper & Vit D deficiency
b) Heat Stress
c) Old Age of hens
d) Defective Shell glands
e) Electrolyte Imbalance
f) Mycotoxins
g) Overuse of Antibiotics

 

Wrinkled Eggs9. Wrinkled Eggs
Reasons:
a) Stress
b) Infectious Bronchitis
c) Defective Shell glands
d) Overcrowding

 

 

 

Pimple Egg10. Pimpled Eggs
Reasons:
a) Eggs with Extra-calcification as granules on the blunt end
b) Old Age Hen
c) Inadequate Nutrition
d) Strain of Chicken

 

 

Calcium Coated Eggs11. Calcium Coated Eggs
Reasons:
a) Defective Shell gland
b) Disturbance during Shell Calcification
c) Excess Calcium in Feed
d) Young hens under any stress usually hold Eggs longer leading to Calcium coated Eggs

 

Mottled Eggs12. Mottled Eggs
Reasons:
a) Disease like Avian Influenza, ND, IB & EDS
b) High Humidity in hen house
c) Mycotoxins
d) Manganese Deficiency
e) Overcrowding
f) Any reason leading to shell fails to dry quickly

 

Broken or Mended Eggs13. Broken or Mended Eggs
Reasons:
a) Stress during Shell calcification
b) Overcrowding
c) Disturbances during Calcification

 

 

Misshapen Egg14. Misshapen Eggs
Reasons:
a) Immature Shell gland
b) Disease like Avian Influenza, ND, IB & EDS
c) Excessive Lighting
d) Stress
e) Overcrowding

 

Spots on Egg Shell15. Spots (White Brown speckled) on Egg Shell
Reasons:
a) Inadequate Nutrition
b) Defective Shell gland
c) Disturbance during Egg calcification
d) Excess Calcium in Feed
e) Stress during Laying

 

For any queries, please email at duttabalaiss@yahoo.co.in 

In an exclusive interview to Poultry TRENDS magazine, Mr. Sanjeev Khanna talks about the current biosecurity & hygiene challenges in poultry industry and his startup – Envizon Biosciences.

Mr. Sanjeev Khanna is an Entrepreneur, Leader & strategist. He has completed his UG from BHU & PG in Business Management from BYB, Mumbai. He has over 25 years of working experience with renowned MNCs like Ranbaxy, Elanco & Novus in various leadership positions with different job responsibilities & geographies. He was instrumental in establishing some of the great companies & brands in Indian subcontinent and spent most of his career span in business development in Livestock and Poultry segment. When he started his journey in Chandigarh, little did he know that this journey will be so exciting and rewarding.

Currently he is working as Managing Director, Envizon Biosciences, a startup based out in Bengaluru and focused on biosecurity & hygiene program in Indian subcontinent. He is adventurous & an explorer who loves traveling, hiking, camping to offbeat places, he is also a health freak & believes that Living a healthy life would extend longevity and regenerate the body and mind.

Excerpts of the interview:

How you describe yourself?

Team player, strategic thinker, grounded & believes in fundamentals to run the businesses & relations. As a professional I got opportunities to explore different geographies and to lead some of the great teams across sub-continent. Working with people with different mindsets and motivations was always exciting and thought provoking. Influencing teams & individuals to explore their true potential and helping them to become successful is always interesting and exciting for me. My strengths lies in people, team building & business development. I always believe that leadership is not a position or title it is an action & example We make leadership decisions every minute of every day, both at work and in our personal lives. When we speak up for what is right, either at work or in our home lives, we are demonstrating leadership.

How did you come with the idea of entering this field?

After working in different segments including Livestock & Poultry for so many years, I finally decided to take different course and started my own venture in mid 2021. I was looking for the key challenges our Industry is undergoing. What are the challenges faced by the producers and how we should provide them science based & trustworthy solutions.

I would like to quote Albert Einstein “ Relativity applies to physics, not ethics”. As in ethics either you are 100% ethical/honest or you are 0% & you cannot be in between place, same theory applies in Biosecurity, either it is 100% or 0% & there is no space in between.

After lot of research and brainstorming, we found that Biosecurity & Hygiene was one of the most over stretched words across the Industry in whole South Asian region, In spite of so many players and almost everyone talking about Biosecurity, still there are huge challenges of disease outbreaks which ultimately shrink the profitability of producers. There is no proven method to validate the claims made by different solution providers either. For example, a product selling at X price & another at 10X have almost same label claims and the producers are in a fix as to what to use and what are the value offered to them. In Envizon Biosciences we invested in machines and equipment to validate the claims to show the value of the solutions with ROI.

What is Envizon Biosciences? Where is it based at?

Envizon Biosciences is a startup company based out in Bengaluru and focused on science, technology & innovation. Our core is new conceptual products which can add value to the customers business. In 2021 Envizon Biosciences entered into an agreement with Intracare NL, a globally renowned company for biosecurity and hygiene products, for marketing of its solution in India. The combination of these leading businesses will offer greater sustainability and broader scope than either alone. In addition to Intracare’s track record of creating successful collaborations, Envizon looks forward to unlocking the shared potential of its combined business strengths with Intracare. By combining Intracare’s state-of-the-art R&D technologies & future proof solutions with Envizon’s diverse distribution network & able workforce, the joint venture will be a category leader in Biosecurity and Hygiene products. Beside that Envizon Biosciences is also working closely with other strategic business partners across the globe to bring latest technologies with good ROI to Indian producers.

What are the specific challenges or obstacles commonly faced when trying to maintain high levels of hygiene by farmers ?

As mentioned above, the biggest challenge we found is lack of right information and validation points. Also, during our research, we didn’t find right equipment and machines which is required to complete the job properly and professionally with many producers. Even with a good product available in the market, if application is not proper, it cannot produce the desired results. The staff who is applying the biosecurity products needs proper and regular training, scientific information & latest updates to use the solutions correctly. Currently many products sold in the market based on relationship & perception, instead of actual scientific data and information.

What is Intra-Hygiene concept?

As you must aware of, in the intensive livestock farming, we are constantly challenged by the threat of pathogens. If pathogens succeed to enter the farm, we are facing production and economical losses, decreased animal welfare, increased mortality, which ultimately leads to higher antibiotic usages & increase the cost. The only way to prevent entry of diseases into the farm is with optimal biosecurity. To mitigate these challenge and help producers to have ultimate biosecurity, Envizon Biosciences for the first time in India, launched a novel concept of Intra Hygiene program for Sheds, Hatcheries and Processing plants with help of latest science-based technologies.

These technologies have been launched first time in India and has two parts – one is the effective solutions, and another is its effective applications with help of the latest machines (mainly imported from Europe) and they play an important role to give full-proof results. All the program is having different validation points to share the results & ROI.

How is Intra-Hygiene different from existing products available in the market?

The current solutions available in Indian market and used by producers are of industrial grade which has been used mostly in the different industries and then started using in the poultry & livestock Industry as well. They are corrosive, not recommended in Livestock Industry and having lot of adverse effects directly or indirectly on the humans & animals, be it in livestock or poultry. All the Intra hygiene solutions used by Envizon biosciences are of food grade & European Union certified, They all are approved by top rated credentials authorities like ECHA, NSF etc. and all the solutions are highly effective with no adverse impact on livestock, machines & equipment.

How do you test the challenge level in a given poultry farm?

As I mentioned, Biosecurity is one of the most overused word across livestock & poultry Industry. People know of it in literal terms only. Washing the farms, spraying disinfectants is not completing the biosecurity cycle and that is the reason we see lot of incidences of disease outbreaks periodically. Then, the biggest challenge in our markets are validation of results.

Envizon Biosciences have a dedicated and certified team for Intra hygiene services. The team is equipped with all the latest scientific equipment to establish the difference and to validate the results before and after the services.

Explain the various stages involved in the process

There are 7 stages involved here in the process to complete the program successfully. Unless we clean the organic materials from the farm, cages, and other specific places, we can not eradicate micro-organisms from the farm. Therefore, we go with different stages including dry cleaning, wet cleaning, foam cleaning, disinfection, and validations at pre & post stages.

Are your products internationally certified? Which other countries is this product being used?

Yes, all the products are internationally certified by international credit rating companies like ECHA, NSF etc under PT01, PT02, PT03, PT04 & PT05. All the products are registered & use in all European, US & Asian countries with extremely satisfied customers. The products are completely safe for the poultry birds, on people who are handling it and on all the expensive machines used in our Industry.

What are the different areas within poultry chain where Intra-Hygiene concept can be applied?

The products can be applied in different poultry chain including Broiler, Layer, Hatcheries, setters & processing units in different dosages. There is no adverse impact of the products as all the products are human grade and safe to use in even food, chicken processing units and even in human hospitals.

What sort of equipment is used for carrying out the task? Do you have a professional team to carry out this task?

Yes, Envizon Biosciences invested in the equipment for proper applications of solutions and we have latest equipment & certified professionals across to do the job successfully.

How has been the market feedback so far? Any big names to share?

We are working on many exciting projects across India and South Asia region. Due to professional ethics & confidentiality agreements, it would not appropriate to share names.

Future depends upon the actions we take today. I do see lot of changes in the producer’s thinking process due to changes in consumer’s behaviour and demand.

India is one of the fastest growing economies in the world & home of 1.4 billion people. With so much development, fast urbanization & youth population, the demand of protein is going to be even higher in coming time. India’s 1Bn young population are well informed, well-travelled and know exactly what they need. With international exposures and social media influences, consumers are now aware and looking for food safety & hygiene while making their choices. This is going to drive our industry and will change the whole scenario and the way it works; the focus is shifting rapidly from production to the processes and safety of the food, which in return is bringing lot of opportunities to the producers to invest in food safety and concepts like Intra Hygiene to achieve their goals.

I am pretty confident that things are changing, and they are changing pretty fast for betterment of our industry.


For any queries, Mr. Khanna can be reached at sanjeev.khanna@envizon.co.in

Poultry Federation of India (PFI) and USA Poultry and Eggs Export Council (USAPEEC) Sign MOU to Address Protein Deficiency

In a historic moment, the Poultry Federation of India (PFI) and the USA Poultry and Eggs Export Council (USAPEEC) signed a Memorandum of Understanding (MOU) to collectively combat protein deficiency and promote the consumption of poultry and poultry products in India.

The signing ceremony at Hotel The Oberoi, New Delhi on March 20, 2024, brought together esteemed representatives from both organizations, including Mr. Greg Tyler, President & CEO USA Poultry & Egg Export Council, Mr. Clay M. Hamilton, Agricultural Minister Counsellor for Agricultural Affairs, USDA, and Mr. Ramesh Khatri, Chairman, Mr. Sanjeev Gupta, Vice President (HQ), Mr. Ricky Thaper, Treasurer, Mr. Parveen Kumar, Vice-President-North Zone and Mr. Jagdish from Poultry Federation of India. This partnership symbolizes a shared dedication to enhancing the nutritional landscape and fostering a healthier, protein-rich future.

Mr. Ricky ThaperMr. Ricky Thaper, Treasurer, Poultry Federation of India highlighted the significance of this partnership in addressing critical nutritional needs and fostering international cooperation in the poultry industry. Mr. Thaper said that this occasion marked the beginning of what promises to be a fruitful collaboration aimed at enhancing nutritional standards and promoting economic growth in the poultry sector.

While addressing the gathering, Mr. Greg Tyler, President & CEO USA Poultry & Egg Export Council added that this collaborative approach encompasses a range of initiatives, including educational, research and development, expansion of market opportunities and campaigns to raise nutritional awareness. PFI and USAPEEC Sign MOUBy combining their expertise, both organizations aspire to raise awareness about the nutritional advantages of poultry products.

Poultry Federation of India Team presented mementoes to Mr. Greg Tyler, Mr. Clay M. Hamilton and Ms. Devna Khanna. Later Ms. Devna Khanna, India Representative of USA Poultry & Egg Export Council, expressed gratitude to all attendees for their support and participation in this milestone event.

This was followed by Trade Reception which provided an opportunity for networking among the invitees including USA Soybean and Corn Grower Farmers, Star Chefs from elite Hotels, Commodity Members, PFI Team and USAPEEC Team.

PFI And USAPEEC Sign MOU

CHESTERFIELD, MO (March 5, 2024) – Novus International, Inc. announces it has completed the acquisition of U.S.-based enzyme company BioResource International, Inc. (BRI).

Novus acquires BRI Under the terms of the agreement, NOVUS becomes the owner of all BRI’s products and intellectual property and takes control of the company’s facilities.

NOVUS President & CEO Dan Meagher pic
NOVUS President & CEO Dan Meagher

“This move will allow us to serve our customers better and expand our innovation pipeline further,” says NOVUS President & CEO Dan Meagher. “Enzymes are vital tools for producers to ensure animal health and well-being and help deliver on-farm profitability. We’re very excited to offer our customers more options, as well as aspire to develop new feed additives.”

The relationship between the two companies isn’t new. NOVUS has partnered with BRI since 2008 to manufacture its protease product, CIBENZA® Enzyme Feed Additive. Meagher says having full ownership and control of the product line and the option to expand NOVUS’ portfolio beyond protease enzymes is a natural fit in the company’s long-term strategic plans.

“As a leader in intelligent nutrition, NOVUS’ priorities include investing further in functional proteins and the gut health segment, growing our portfolio, and achieving stronger control of our supply chain,” he says. “Along with supporting these goals, acquiring BRI also increases our capabilities to develop innovative solutions in the fermentation space.”

BRI’s products include Versazyme® protease feed additive, Xylamax® xylanase feed enzyme, Dymanase® mannanase enzyme, Phytamax® granulated, thermostable, microbial 6-phytase enzyme; and EnzaPro® enzyme and direct-fed microbials, among others.

BRI’s co-founder and CEO Giles Shih
BRI’s co-founder and CEO Giles Shih

BRI’s co-founder and CEO Giles Shih, Ph.D., says the acquisition and tapping into the knowledge of employees in both BRI and NOVUS allows for the continued growth of the company he helped create 25 years ago.

“We are super excited about the future where BRI’s products and people combine forces with the global reach and deep industry knowledge NOVUS brings to the feed additive space,” Shih says. “This deal is not one of happenstance. Our two companies have worked closely together since 2008 to launch the first generation of heat-stable protease feed enzymes and shape how they are developed, marketed, and optimized to add value to customers worldwide. This acquisition will enable NOVUS to innovate the next generation of proteases and enzymes that will do even more, from promoting gut health to promoting the bottom line.”

Along with BRI’s current product portfolio, NOVUS also takes over the company’s facilities in North Carolina in the U.S.

Meagher says continuity is key for current BRI customers and they should experience “business as usual” during the integration process.

NOVUS is the intelligent nutrition company providing solutions for the global animal agriculture industry. The company’s portfolio includes bis-chelated organic trace minerals, enzymes, organic acids, essential oils, liquid and dry methionine, and a network of experts worldwide to provide guidance on management best practices. NOVUS is owned by Mitsui & Co., Ltd. and Nippon Soda Co., Ltd.

For information on how NOVUS is supporting producers around the world, visit novusint.com.

Source: Poultry TRENDS magazine

Dr. Anju Ajay Deshpande is a veterinarian turned into an entrepreneur. She completed BVSc. from Nagpur Veterinary College and MVSc. in VPH from COVAS Mannuthy. She has worked at various levels, her journey started as veterinary biosecurity officer, as of today she is the director of various organisations engaged in poultry business.

In an exclusive interview to Poultry TRENDS magazine, Dr. Anju shares about her journey, challenges, accomplishments and her message to women.

Q: Please brief us about your background

Dr. Anju Deshpande: My parents were government employees and during my growing up days, I was fortunate to travel pan India gaining exposure of places and things. I realised the huge scope in veterinary science and work offering more flexibility than medical field. Veterinary Public Health specialisation was one of the hottest things, as it is a bridge between human health and animal health science. At the time, there were not many openings for epidemiologists or animal-based food industries in India though. I chose the poultry industry, which was just getting off the ground. However, to gain experience, there were very few poultry companies offering jobs to lady veterinarians.

Q: How was your job as a poultry veterinarian

Dr. Anju Dr. Anju Deshpande: In 1995, I joined United Hatcheries Pvt. Ltd. Hyderabad. I may have been the only lady veterinarian in India who not only worked but also stayed at the poultry breeding farm. It was not considered a white-collar job then, and even now very few professionals opt for this job, because, usually breeding farms are in remote places, far away from the nearest village with harsh conditions. However, it was there that I learned the very basics of the poultry industry. After that I worked for Pure Line Poultry Breeding farm too in Tarkeshwara Hatcheries Pvt. Ltd., Nashik.

Later I worked in various positions that required a variety of skills – as an LDO extension, a Lecturer for the State Level Training Centre for officers. After completing “Poultry Viral Vaccine Production Certification” in IVRI, I worked for the Institute of Veterinary Biologicals Pune.

Q: When did you get the idea to start a business and how you started?

Dr. Anju Deshpande: Since my first job, I always thought about the fact that while many veterinary doctors were employees in the poultry industry, and in spite of being domain experts, why they couldn’t become businessmen. But now I understand that only few have the courage to burn their ships. I always had a dream of having my own business someday, to create employment for myself and others. Big dreams, though without any resources or experience.

Dr. Anju at workDuring my second job in Tarkeshwara Hatcheries, I met Ajay who was working as Production Manager. Ajay is BVSc from College of Veterinary and Animal Sciences, Parbhani and also my batch mate. As part of his job, he was required to remain in remote area without basic facilities, be it a hospital, market or local transport. I started discussing my business ideas with him as even before we got married, my ideas about creating our own business had started taking shape. However, I was only able to work towards it when I was selected for a government job through MPSC.

We then decided that instead of me, my husband could start business and I would support him in initial stages and later, join the business. This was the time when our first child was 2 years old.

In 2003, we started with a small broiler breeder farm with the help of mentors and well-wishers. Our first child was 6 and second was 2 by then.

Q: How did you contribute and support the new venture?

Dr. AnjuDr. Anju Deshpande: In spite of being in a government job, I was one of the founder members in our company. I was always closely involved in policy matters, as we were investing almost every penny of our life-savings there. Being a permanent government employee and a class one officer, I was always the guarantor for the business loans we required.

I started by learning new skills, like accounting, preparing balance sheets, understanding taxes helping him, going to farm on holidays. Discussing practical problems and finding solutions till late waking hours was our routine for years. Even our children’s picnics were always only on poultry farms. Long drives to attend relatives or visiting family was opportunity to discuss more about our experiences with the poultry business. We couldn’t have achieved the goal without each other as team.

Q: What were challenges as first generation in business?

Dr. Anju Deshpande: Major challenges came forth in facing and losing everything at the time of first Bird flu pandemic. Rebuilding from zero was quite an experience; we supplied eggs and sprouts and vegetable to marts to retain our staff in pandemic period.

As a government officer I had to work hard and travel to remote villages to support my family for 15 long years, until our business could support me. Those years weren’t as difficult as waiting for my aspiration; to be able to work full time for my own business; I wanted to explore new ideas, so, letting-go of golden opportunities was a challenging thing for me. In those days, the private sector was growing and everyone was busy, it was difficult seeing the world pass by, while we were simply making ends meet. Whatever money we earned and saving through sacrificing on small things was invested in the business. And, the year Ajay paid his personal tax as much as my salary, I felt free to resign from my government job. By that time, I had some minor health issues, but finally I left the government job, leaving the tension and the highly sought pension.

Q: How did you overcome your hurdles?

Dr. Anju Deshpande: Each hurdle was dealt with as it came but generally speaking, we prepared ourselves for hurdles by anticipating. By thinking ahead and planning, sticking to the agenda, and living for the dream. Other things helping cope up were hard-work of course and keeping personal expenses to a minimum.

I was always prepared so that whenever opportunities presented, we were ready to commit. Avoiding toxic relations helped maintain energy levels to cross hurdles. We kept our morale high, encouraging and appreciating each other, acknowledging the efforts and celebrating small achievements. All is easier said than done. I must say hurdles were easier only because we helped each other in work and at home too. At times Ajay babysat without complain plus my mother always rushed whenever we needed. These things helped me a lot while raising kids.

Q: Where do you find yourself today, tell us about your accomplishments?

Dr. Anju DeshpandeDr. Anju Deshpande: I have planted and nurtured more than 500 trees on our farms, the pleasure is immeasurable. We have 400 employees and that gives me immense happiness.

Besides that currently, I am one of the four directors at Siddhivinayak Poultry Breeding Farm and Hatcheries Pvt. Ltd. The company now has 1 lakh broiler breeders and placement of 20 lakh chicks per month in contract farming.

I am also a director and partner in Avian Research & Development Pvt. Ltd, where my focus is on creating marinated chicken products.

My other company is Percept-Accuity Techno-soft Pvt. Ltd, which specializes in an ERP software designed for all aspects of the poultry industry.

Q: What are your strengths?

Dr. Anju Deshpande: I am focused about my goals. I am aware of what I do want. I appreciate everyone for who they are and cherish every moment. While I am most of the times positive for the future, even in case of failure, I feel like I have given it a good throw and the journey was worth it.

Q: What are you passionate about?

Dr. Anju Deshpande: I am a very passionate person. I take all my responsibilities seriously, whether it is professional work, business and ambience, creating, learning and teaching, or managing my home and cooking for my loved ones. I am usually engrossed in learning new things which I do by reading, watching and keenly observing people and processes.

I share professional and life skills knowledge selflessly. I love to train people for the technical knowhow and soft skills that are essential for optimum performance and dynamic involvement in work. I am happy to delegate work to my teammates. I believe everyone has talent and value, if given space and time. I also love multi-tasking and managing my time. The most rewarding thing for me is finish the work in hand and still have ample time, to explore new things.

Q: What does Power mean to you?

Dr. Anju Deshpande: For me, power is within. It means to be self-sustained, able to survive in all odds and grow big and grow wise. Being strong enough to support others is a mental power. Any individual doing that is powerful.

Q: What are the prerequisites for women to do business?

Dr. Anju Deshpande: Love is the force of all creations and manifestations, love for business is basic. Besides this, knowledge of the sector, deep desire to win, perseverance, courage to risk it all, basic skill-sets and strong support systems to depend on are some prerequisites. Encouragement, appreciation and nurturing is needed for anything to take root and grow.

Q: Any message for women?

Dr. Anju Deshpande: I have three messages for all the women:

Dr. Anju Deshpande

  • Firstly, know yourself; you are already empowered by natural resilience and complex brain wirings. Women work shoulder to shoulder with men, but hesitate to come in the limelight. My message is “be assertive and learn to take credit of your own work proudly and gracefully, and never let anyone brush you or your words aside”.

  • Second, I feel that every lady should understand the synergetic power of yin and yang. Successful women are successful often because they got support somewhere, from their father, brothers, husband, sons, friends, sometimes from all of them. When necessary, for your pride, fight it out, but don’t be mean to men, cherish and love if you have them in your life.

  • Lastly, show your man how to support you at home and in the kitchen, it doesn’t make you weak, but it makes him strong.

Dr. Anju Deshpande can be connected at LinkedIn

The respiratory system is essential to the birds’ performance. It is important not only for oxygen transport, but also for removal of metabolic waste and by-products. A healthy respiratory system can eliminate 80% of the germs inhaled within 1-3 hours. Due to its highly efficient nature and exposure to the external environment, it is easily susceptible to a range of diseases and problems which can insidiously affect the genetic potential of performance livestock.

Sukhjeet Singh Kahlon– Technical Manager, Intracare B.V. The Netherlands

In winters and times of respiratory distress and discomfort, animals spend less time eating and drinking. Essential oil-based products with their unique blend have long been used in livestock farming not only to help alleviate the severity and duration of respiratory problems, but also to help keep animals cool during thermal challenges.

Just mixing of some oils at any concentration does not make an ideal product it requires deep know-how to find a fine balance between active ingredients and supporting elements. When a group of animals require treatment, drinking water application is a responsible and useful option on the condition that the drinking water, the system, and the products are of good quality. Products should be selected which are carefully formulated to meet an optimal balance between solubility, stability, and bioavailability. The active ingredients should be well soluble and highly available to the animal. The supporting elements in formulations help to create a homogenous mixture.

Aerosol advt
Advertisement

With this knowledge in hand, very few products have therefore been optimally formulated to be fully soluble in water and is also very well dispersed in on-farm conditions. The product must be immediately solubilized in water upon addition or injection and is fully homogenous in solution. If added to a bulk tank, minimal stirring needs to be required and does not require much external energy inputs such as a mechanical mixer. The selected product should not require the use of warm or hot water to be fully solubilized, as this is oftentimes not practical on the farm. Being completely soluble and consistently dispersed in water, it should have less risk of encouraging biofilm growth and the risk of drinking nipple blockages is also very much reduced.

With its highly soluble and homogenous dispersion properties, product should be selected based on following criterion to helps farmers to:

1 Reduce labour: Selected product can be directly dosed to the drinking system and doesn’t require warm water and/or a pre-solution step. This valuable time can then be used by the farmer for other important management practices.

2 Equal distribution in the drinking water: Homogeneity and uniformity of the flock is an important factor for producers. Therefore, it is equally important that supplementation is also homogenous and uniformly dispersed in the drinking water, reducing the risks of stragglers and non-uniform growth.

3 High quality, well soluble and stable in solution to prevent biofilm: The Selected product should not only be well soluble but should also stay stable in solution. This not only helps to prevent biofilm formation, and the animals consume a continuous, consistent dose thus reducing risks of taste-aversion and decrease in water consumption.

Figure 1: Product A dispersed fully in water (20°C), right – product B with obvious clump forming after dosing in water (20°C)

A lab-scale comparison was conducted to determine the solubility and dispersion aspects of two leading brands from Europe in room temperature water of 20°C. Product-A and Product-B were dropped into tap water at the advised dosages according to the labels. It was observed that product-A had perfect dispersion and did not form clumps or sediments when compared to other competitor products. Even gentle stirring did not manage to completely break the clumps of competitor products and in a drinking line. This could surely lead to nipple blockage and uneven dosing causing an eventual headache for the farmer.

In conclusion, it is important for a drinking-water based nutritional solution to not only be functional, but also have well soluble properties with good homogenous dispersal at farm conditions.

Avian mycoplasmosis, is a contagious respiratory disease that affects poultry, particularly chickens and turkeys. It is caused by various Mycoplasma spp. and can lead to significant economic losses in the poultry industry. Mycoplasmosis is primarily caused by two species of Mycoplasma: Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS). These bacteria are responsible for a range of respiratory and systemic infections in birds. These are simplest prokaryotic microorganisms with the ability of self-replication, the most distinguishing characteristic of these bacteria is lack of cell wall. MG is the most common cause of mycoplasmosis in poultry and infected chicken shows a wide variety of symptoms including rales, coughing, nasal discharge, conjunctivitis, reduced feed efficiency, air sacculitis and egg production typically declines with initial infection, then recovers and maintained at a lower level.

AuthorsMycoplasma gallisepticum infection in birds usually takes a long time to manifest and is followed by severe respiratory tract inflammation. In flocks, the disease often goes unnoticed and causes latent infections. According to earlier studies, conditions including high feed density, heat and cold stress, high levels of ammonia, accumulation of feces, fouling of the chicken house, wide temperature variations, and abrupt climatic changes can all contribute to the spread and outbreak of disease.

Virulence factors

Variety of surface polypeptides and lipoproteins of MG plasma membrane are known and have putative function in motility, cytadhesion, surface antigen variation, and nutrient acquisition, all of which are essential virulence factors for MG.

Host- Pathogen interaction

M. gallisepticum attaches itself to the ciliated cells in the respiratory tract of birds using specialized surface proteins. For effective colonization and eventual pathogenesis, the attachment of MG to host cell is utmost important. Once attached, it invades the host cells. The gliding motility of Mycoplasma gallisepticum organisms enables them to enter target tissues and overcome host’s physical defences such respiratory mucus and ciliary activity.

Colonization and Multiplication: Numerous MG cytadhesins and putative cytadhesins have a recognized role in emergence phenotypic variation, which is assumed to be a key virulence component since it appears to facilitate chronic infection and evasion of host immune response. After invading the cells, the bacterium replicates within them. This leads to damage to the host cells, interfering with their normal function.

Inflammatory response: The host’s immune system recognizes the presence of the bacterium and mounts an inflammatory response. This involves the release of various immune cells and inflammatory mediators to combat the infection.

Tissue Damage and Lesions: The inflammation, coupled with the bacterial replication and host immune responses, can lead to damage in the respiratory tissues. This damage may manifest as lesions in the trachea, bronchi, and air sacs.

Transmission

M. gallisepticum can spread within a flock through respiratory secretions, faeces, and contaminated equipment or environment. Some wild bird species, multiage commercial layer flocks, and backyard flocks are potential MG infection reservoirs. The upper respiratory tract and conjunctiva are portals of entry for organism in aerosols or droplets.

The dynamics of MG infection relies heavily on clinically or sub clinically infected carrier birds since Mycoplasma gallisepticum hardly survives outside the host for longer than a few days, yet several studies reported the ability of MG to survive up to several days on contaminated fomite materials (dust, feathers etc) provides important insights on epidemiology of the disease. Some M. gallisepticum strains capacity to develop biofilms may enable them to survive in the environment for longer duration. MG can be transmitted from infected breeder flock to their progeny via transovarian transmission. Some studies concluded that the vertical transmission of MG occurs at the highest rates during the acute phase of disease when the level of MG is at peak in respiratory tract and declines subsequently as the post infection interval lengthens.

Carrier State: Some birds may become carriers of M. gallisepticum, meaning they harbour the bacterium without showing overt signs of illness. These carriers can serve as a source of infection for other birds.

Symptoms

Respiratory Distress: Birds infected with mycoplasmosis often exhibit respiratory distress, characterized by coughing, sneezing, nasal discharge, and wheezing.

Reduced Egg Production: In laying hens, mycoplasmosis can lead to a decrease in egg production, as well as a decrease in egg quality.

Swollen Eyes and Sinusitis: Infected birds may develop swollen eyes and sinusitis, along with a discharge from the eyes.

Conjunctivitis: The infection can lead to inflammation of the conjunctiva, causing redness and discharge from the eyes.

General Weakness: Infected birds may exhibit lethargy, decreased appetite, and a drop in overall activity levels.

Diagnosis

Accurate diagnosis of mycoplasmosis is crucial for effective management. It is typically achieved through a combination of clinical signs, post-mortem examinations, and laboratory tests. These tests may include serology (blood tests), PCR (polymerase chain reaction), and bacterial isolation from affected tissues.

Prevention and Control

Biosecurity Measures: Implement strict biosecurity measures to prevent the introduction and spread of mycoplasmosis. This includes limiting visitor access, maintaining separate footwear and clothing for workers, and disinfecting equipment and facilities regularly.

Vaccination: There are vaccines available for both MG and MS, determine the most appropriate vaccination schedule for your poultry.

Cleanup Programs: Use of appropriate molecule for effective cleaning up of mycoplasmal infection prior to vaccination may provide better results.

Minimize Stress: Stress weakens the immune system, making birds more susceptible to infections. Provide a low-stress environment by ensuring proper nutrition, ventilation, and living conditions.

Surveillance: Regularly monitor your flock for any signs of illness. Early detection allows for prompt intervention and reduces the spread of the disease.

Authors:

Reference:
Beaudet, J., E. R. Tulman, K. Pflaum, X. Liao, G. F. Kutish, S. M. Szczepanek, L. K. Silbart, and S. J. Geary. 2017. Transcriptional profiling of the chicken tracheal response to virulent Mycoplasma gallisepticum strain Rlow. Infect. Immun. 85: e00343- 17.
Browning, G.F., A.H. Noormohammadi, and P.F. Markham. 2014. Identification and characterization of virulence genes in mycoplasmas. In: Mollicutes – Molecular Biology and Pathogenesis. G.F. Browning and C. Citti, ed. Caister Academic Press, Norfolk, UK. 77–90.
Boguslavsky, S., D. Menaker, I. Lysnyansky, T. Liu, S. Levisohn, R. Rosengarten, M. Garcia, and D. Yogev. 2000. Molecular characterization of the Mycoplasma gallisepticum pvpA gene which encodes a putative variable cytadhesin protein. Infect Immun. 68:3956–3964.
Evan, J. D., S. A. Leigh, S. L. Branton, S. D. Collier, G. T. Pharr, and S. M. D. Bearson. 2005. Mycoplasma gallisepticum: current and developing means to control the avian pathogen. J. Appl. Poult. Res. 14:757–763.
Hochachka, W. M., and A. A. Dhondt. 2000. Density-dependent decline of host abundance resulting from a new infectious disease. Proc. Natl. Acad. Sci. USA. 97:5303–5306.
Indikova, I., M. Vronka, and M.P. Szostak. 2014. First identification of proteins involved in motility of Mycoplasma gallisepticum. Vet Res. 45:99.
Ishfaq, M., W. Zhang, W. Hu, S. Waqas Ali Shah, Y. Liu, J. Wang, Z. Wu, I. Ahmad, and J. Li. 2019. Antagonistic effects of Baicalin on mycoplasma gallisepticum-induced inflammation and apoptosis by restoring energy metabolism in the chicken lungs. Infect. Drug Resist. 12:3075–3089.
Jan, G., C. Brenner, and H. Wroblewski. 1996. Purification of Mycoplasma gallisepticum membrane proteins p52, p67 (pMGA), and p77 by high‐ performance liquid chromatography. Protein Expr Purif. 7:160–166.
Keeler, C.L., Jr., L.L. Hnatow, P.L. Whetzel, and J.E. Dohms. 1996. Cloning and characterization of a putative cytadhesin gene (mgc1) from Mycoplasma gallisepticum. Infect Immun. 64:1541–1547. 130.
Ley, D. H. 2003. Mycoplasma gallisepticum infection. Pages 722–743 in Diseases of Poultry. Y. M. Saif, ed. 11th ed. Iowa State Press, Ames, IA.
Mikaelian, I., D.H. Ley, R. Claveau, M. Lemieux, and J.P. Berube. 2001. Mycoplasmosis in evening and pine grosbeaks with conjunctivitis in Quebec. J Wildl Dis. 37:826–830.
Miyata, M. and D. Nakane. 2014. Gliding mechanism of the Mycoplasma pneumoniae subgroup. In: Mollicutes – Molecular Biology and Pathogenesis. G.F. Browning and C. Citti, eds. Caister Academic Press, Norfolk, UK. 237–253.
Raviv, Z., S. Callison, N. Ferguson‐Noel, V. Laibinis, R. Wooten, and S.H. Kleven. 2007. The Mycoplasma gallisepticum 16S‐23S rRNA intergenic spacer region sequence as a novel tool for epizootiological studies. Avian Dis. 51:555–560.
Szczepanek, S.M. and L.K. Silbart. 2014. Host immune responses to mycoplasmas. In: Mollicutes – Molecular Biology and Pathogenesis. G.F. Browning and C. Citti, eds. Caister Academic Press, Norfolk, UK. 273–288.
Zimmermann, C.U. 2014. Current insights into phase and antigenic variation in mycoplasmas. In: Mollicutes – Molecular Biology and Pathogenesis. G.F. Browning, and C. Citti, eds. Caister Academic Press, Norfolk, UK. 165–196.